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ampl43 plasmid  (Addgene inc)


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    Addgene inc ampl43 plasmid
    Ampl43 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ampl43+plasmid/pmc12505964-106-26-28?v=Addgene+inc
    Average 93 stars, based on 4 article reviews
    ampl43 plasmid - by Bioz Stars, 2026-07
    93/100 stars

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    ampl43 plasmid  (Addgene inc)
    93
    Addgene inc ampl43 plasmid
    Ampl43 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ampl43+plasmid/pmc12505964-106-26-28?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    ampl43 plasmid - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier
    ampl43  (Addgene inc)
    93
    Addgene inc ampl43
    a Schematic of <t>amPL43</t> expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.
    Ampl43, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ampl43+plasmid/pmc07695836-359-0-5?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    ampl43 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

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    a Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Journal: Communications Biology

    Article Title: An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology

    doi: 10.1038/s42003-020-01452-9

    Figure Lengend Snippet: a Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Article Snippet: AmPl43 was also deposited to Addgene (Addgene ID: 161830).

    Techniques: Expressing, Plasmid Preparation